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Fil: Application-brief-What-can-you-do-with-a-revio-system.pdf
Vedlegg til anbudet Forbruk til Packbio sekvensieringssystem fra Universitetet i Oslo.
Application brief
WHAT CAN YOU DO WITH A REVIO™ SYSTEM?
The outstanding accuracy of HiFi long-read sequencing allows you to get the most out of each Revio run. Discover how
you can access more information at reduced cost and higher throughput for each of your sequencing projects.
• HiFi sequencing provides reliable answers through exceptional accuracy, with 90% of bases Q30+. Maximize genetic
information and detect more variant types including direct methylation in native molecules.
• Perform on-board primary analysis including barcode demultiplexing, methylation calling, and Google DeepConsensus.
• Optimize your workflow with simplified consumables and rapid run setup. Take advantage of four independent sequencing
stages to perform multiomic experiments in one sequencing run.
• Generate a $1,000 complete, phased human genome.*
More samples, more discovery
Samples per Revio run
Application Samples per SMRT Cell* using 4 SMRT Cells*
Whole genome sequencing
De novo assembly 1 4
Variant detection Structural variants: 3 Structural variants: 12
All variants: 1 All variants: 4
Microbial de novo assembly 96 384
RNA sequencing
Kinnex single-cell transcriptomics 1 4
Kinnex full-length RNA 4 16
Targeted sequencing
Amplicon sequencing ≥1,000 ≥4,000
Target enrichment 20 Mb panel: 12 20 Mb panel: 48
2 Mb panel: 72 2 Mb panel: 288
100 kb panel: 288 100 kb panel: 1,152
Metagenomics
Shotgun metagenomic profiling 96 communities 384 communities
Shotgun metagenomic assembly 12 communities 48 communities
Kinnex 16S rRNA 1,536 communities 6,144 communities
* All sample throughputs are estimates per Revio run using 1 or 4 SMRT Cells. Coverage may vary based on sample quality, library quality, and fragment lengths. Currently available
SMRTbell® barcoded adapter plate 3.0 contains 96 SMRTbell barcoded adapters. Whole genome sequencing for a 3 Gb human-like genome at >15× per haplotype for de novo assembly,
>10× coverage for structural variants, and >30× coverage to detect more variants. Microbial de novo assembly assumes microbes with ~1.2 Gb of total genome size per SMRT Cell at >50×
per sample. Single-cell transcriptomics assumes ≥80 million reads per library. Full-length RNA assumes a total of 40M reads regardless of plexity. Amplicon sequencing assumes 12-hour
movie time for 1–5 kb, 24-hour movie time for 5+ kb, and >50× per sample. Target enrichment assumes >50× per sample.
From sample prep to analysis, PacBio® has you covered
Sample prep Library prep Sequence Analysis
Nanobind® DNA kits enable SMRTbell prep kit 3.0 The Revio system adds On-board SMRT® Link
extraction of HMW gDNA supports manual and affordability, high throughput, primary analysis, methylation
from a variety of sample automated library prep for and ease of use to a calling, and Google
types effortless SMRTbell library foundation of long reads, DeepConsensus
creation exceptional accuracy, and
direct methylation
Ordering information
Part number Product Description List price (USD)
102-090-600 Revio system Sequencing instrument $779,000
102-817-600 Revio polymerase kit Reagents for binding polymerase to 24 SMRTbell libraries $1,800
102-587-400 Revio sequencing plate Sequencing reagents supporting 4 SMRT Cells $720
102-202-200 Revio SMRT Cell tray Tray of 4 Revio SMRT Cells $2,960
102-817-900 Revio reagent kit, 24-pack 1 Revio polymerase kit, 6 Revio sequencing plates, $23,880
and 6 Revio SMRT Cell trays
Connect with PacBio Learn about application- Learn more about the Revio system
North America: [skjult]@pacb.com specific workflows pacb.com/revio
South America: [skjult]@pacb.com pacb.com/applications
EMEA: [skjult]@pacb.com
Asia Pacific: [skjult]@pacb.com
Research use only. Not for use in diagnostic procedures. © 2023 Pacific Biosciences of California, Inc. (“PacBio”). All rights reserved. Information in this document is subject to change without notice. PacBio
assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of PacBio products and/or third-party products. Refer
to the applicable PacBio terms and conditions of sale and to the applicable license terms at pacb.com/license. Pacific Biosciences, the PacBio logo, PacBio, Circulomics, Omniome, SMRT, SMRTbell, Iso-Seq,
Sequel, Nanobind, SBB, Revio, Onso, Apton, and Kinnex are trademarks of PacBio.
102-326-578 REV02 30NOV2023
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